Lloyd Fraser Group has won a five-year contract to collect and distribute fresh milk for Milk Link.
The transport firm's operation will be based in Milborne St Andrew, Dorset, collecting from farms and distributing to Milk Link customer facilities in the south of England.
Milk Link is the UK's largest producer of cheese, and also manufactures creams and flavoured milks.
Keith Spencer, Lloyd Fraser divisional director, says: "To become their logistics contractor of choice in the Dorset area and to be able to expand with them in Southern England represents a significant opportunity for our group".